METHOD FOR PRODUCING TRANS-RESVERATROL USING MAGNETIC PHOTOSYNTHETIC MEMBRANE VESICLE

摘要

The present invention relates to a method for synthesizing trans-resveratrol in vitro by using a refined enzyme. The present invention has established a biosynthesis pathway that produces trans-resveratrol, using 4-coumaric acid and malonic acid as substrates by combining 4-coumarate-coA ligase, malonyl-CoA synthetase, and stilbene synthase. In addition, the present invention has completed the overall reaction by further combining adenylate kinase, pyrophosphatase, and a magnetic photosynthetic membrane vesicle for recycling the co-enzyme required for an enzymatic reaction. Furthermore, to increase the efficiency of enzyme usage and reliable obtainment of reaction products, the overall reaction was carried out while each reaction enzyme is immobilized and sealed by a mesh, and trans-resveratrol was produced with a production yield of 1.06 g/L/day. In addition, the final product, trans-resveratrol, can be separated as precipitates, thereby increasing the possibility of industrial application.;COPYRIGHT KIPO 2019