Cas9 A method for regulation of gene expression by expressing Cas9 protein from the two independent vector

摘要

The present invention provides a method of controlling genes, comprising introducing into a cell a recombinant vector expressing a first domain comprising a N-terminus of a Cas9 protein and a recombinant vector expressing a second domain comprising a C-terminus, respectively. The present invention relates to a composition comprising the recombinant vector, a kit for controlling gene expression, and a method for intracellular production of Cas9 protein. The present invention further relates to a composition comprising a viral vector packaging the first domain and a transformed cell into which the viral vector packaging the second domain is introduced and a virus produced therefrom.