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A method for producing stem cell clones suitable for inducing differentiation into somatic cells.

机译:一种产生适于诱导分化为体细胞的干细胞克隆的方法。

摘要

Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).
机译:提供了一种产生干细胞克隆的方法,包括以下步骤:(i)将与诱导分化为体细胞有关的外源基因引入干细胞;(ii)诱导引入外源基因的干细胞分化为体细胞;(iii)使分化诱导的体细胞去分化;和(iv)从步骤(iii)中形成的干细胞菌落中分离出具有掺入其染色体的外源基因的干细胞。

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