AUXILIARY PLASMID LENTIVIRAL EXPRESSION VECTOR FOR OBTAINING HIGH TITERS OF Vpx-CONTAINING LENTIVIRAL PARTICLES, WHICH PROVIDES EFFECTIVE INFECTION OF HUMAN MONOCYTES AND DENDRITIC CELLS

摘要

FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology and can be used in therapy of oncological diseases. Described is a universal auxiliary plasmid expression lentiviral vector for obtaining high titres of viral particles containing a Vpx gene, comprising as basic elements providing the functioning of a vector, a virion-associated Vpx protein sequence containing a C-terminal HIV-1 gag/pol motif and a FLAG sequence and combined with a transactivator protein Rev gene sequence through a specific T2A sequence which provides cleavage of the fusion proteins during the translation of the mRNA transgene; as regulatory elements – RSV promoter/enhancer (rous sarcoma virus) and NES (nuclear export signal) – export signal from nucleus to cytoplasm.EFFECT: disclosed is an auxiliary plasmid lentiviral expression vector for obtaining high titers of Vpx-containing lentiviral particles, which provides effective infection of human monocytes and dendritic cells.1 cl, 4 dwg, 1 tbl, 3 ex