Recombinant plasmid pHis6-flagG-protE, providing the synthesis of a recombinant chimeric protein including tick-borne encephalitis virus glycoprotein E epitopes and S. typhii flagellin G and used as a basis for tick-borne encephalitis virus vaccine

摘要

FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Described is a recombinant plasmid pHis6-flagG-protE with built-in fliG and TBEVgp1 genes coding hybrid recombinant flagG-protE protein inducing an immune response to tick-borne encephalitis virus.EFFECT: invention provides production of recombinant chimeric protein without inclusion bodies formation, provides synthesis of recombinant chimeric protein having amino acid sequence (SEQ ID NO: 2), including epitopes of glycoprotein E of tick-borne encephalitis virus and flagellin G Salmonella typhii and used as a base for vaccine against tick-borne encephalitis virus.1 cl, 5 dwg, 2 tbl, 4 ex