STRAIN ESCHERICHIA COLI PRODUCER OF RECOMBINANT PROTEIN TSR A CHOLERIC VIBRION BIOVAR EL TOR

摘要

FIELD: biotechnology.SUBSTANCE: invention refers to biotechnology and genetic engineering. Disclosed is a method of producing E. coli BL21 Star™(DE3) pET302/NT-His tcpA – producer of recombinant protein TcpA cholera vibrio biovar El Tor. Method provides for transformation of E. coli BL21 Star™(DE3) strain cells plasmid pET302/NT-His containing a cloned tcpA gene fragment with size of 534 base pairs from plasmid pCR2.1. tcpA, obtained by PCR using oligonucleotide primers F ccgctcgaggattcgcagaatatgactaaggctgc and R gctggatccttaactgttaccaaaagctactgtgaat.EFFECT: invention provides high-yield TcAA protein.1 cl, 1 ex