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METHOD OF OBTAINING CHITIN FROM HERMETIA ILLUCENS BLACK SOLDIER FLY LAVRAE

机译:从Hermetia illucens黑色士兵蝇幼虫中获得甲壳素的方法

摘要

FIELD: biochemistry.;SUBSTANCE: invention is aimed at obtaining high-molecular chitin from the Hermetia illucens black soldier fly larvae. Method of obtaining chitin from the Hermetia illucens black soldier fly larvae involves the processing of immobilized larvae on the oil press. To 30–50 g of the obtained solid residue, add 150–200 ml of 85 % phosphoric acid, mix thoroughly and leave for 16–20 hours at room temperature. Resulting suspension is filtered under vacuum through a No. 1 glass porous filter and washed with 70–100 ml of 40 % phosphoric acid. Combined filtrate is diluted with 2.5–3.0 L of water with vigorous stirring. Precipitate of chitin in the filtrate is washed with water by the method of decanting to a pH of 5.5–6.0 and freeze dried.;EFFECT: invention makes it possible to simplify the process of obtaining chitin from the Hermetia illucens black soldier fly larvae, and also to obtain chitin in amorphous form.;1 cl, 3 ex
机译:领域:生物化学。物质:本发明旨在从Hermetia illucens黑战士蝇幼虫中获得高分子甲壳质。从Hermetia illucens黑战士苍蝇幼虫获得甲壳质的方法包括在油压机上加工固定化的幼虫。向获得的30–50 g的固体残留物中,添加150–200 ml的85%磷酸,彻底混合并在室温下放置16–20小时。所得悬浮液在真空下通过1号玻璃多孔过滤器过滤,并用70-100 ml的40%磷酸洗涤。合并的滤液在剧烈搅拌下用2.5–3.0 L的水稀释。通过倾析至pH 5.5-6.0的方法用水洗涤滤液中的几丁质沉淀,然后冷冻干燥。效果:本发明可以简化从Hermetia illucens黑战士蝇幼虫中获得几丁质的过程,并且也获得无定形形式的几丁质。; 1 cl,3 ex

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