TEST SYSTEM FOR DETECTION OF THE VIRUS GENOME OF PARAINFLUENZA 3 TYPES AT CATTLE WITH A MULTIPLEX POLYMERASE CHAIN REACTION WITH FLUORESCENT DETECTION IN REAL TIME MODE

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to biotechnology, and in particular to means of diagnosing parainfluenza virus type 3 in animals. Test system for detecting the genome of the causative agent of coronavirus infection in animals using multiplex polymerase chain reaction with fluorescence detection in real time is proposed. Test system includes a buffer for carrying out the polymerase chain reaction, primers and fluorescent probes specific for Coronavirus A and for the internal control sample, a mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE and reverse transcriptase MMLV REVERSE TRANSCRIPTASE, buffer for diluting RNA in the form of deionized water, internal control sample, negative control sample, positive control sample, for internal control sample – bacteriophage MS2 suspension, and for a positive control sample, a mixture of recombinant plasmid DNA containing a fragment of the BPI 3 virus genome and a fragment of the bacteriophage genome is used.;EFFECT: invention serves to extend the functionality and obtain reliable diagnostics.;1 cl, 5 tbl, 1 ex