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FLU VIRUS PROTEINS PRODUCER MDCK CELL (OPTIONS)

机译:流感病毒生产者MDCK细胞(可选)

摘要

FIELD: biotechnologies.;SUBSTANCE: invention relates to the biotechnology. Described is the MDCK, the influenza virus protein producing cell, containing a lentiviral plasmid vector pLenti6.3/TO/HA(H1N1)-M1-NA, containing influenza virus (H1N1) protein the hemagglutinin, neuraminidase and M1 genes, shown in fig. 3. Described is the MDCK, the influenza virus protein producing cell, containing a lentiviral plasmid vector pLenti6.3/TO/HA(H3N2)-M1-NA, containing the cloned hemagglutinin gene from the H3N2 subtype strain, as well as the neuraminidase and the M1 protein genes of the (H1N1) subtype strain shown in fig. 2. Described is the MDCK, the influenza virus protein producing cell, containing a lentiviral plasmid vector pLenti6.3/TO/HA(B-)-M1-NA, containing the influenza B virus hemagglutinin gene, as well as the neuraminidase and the M1 protein genes of the (H1N1) influenza virus, shown in fig. 1.;EFFECT: influenza virus MDCK producing cell is used for the influenza virus the virus-like particles (HPV) development.;11 cl, 23 dwg, 6 ex, 10 tbl
机译:技术领域:本发明涉及生物技术。描述了MDCK,即流感病毒蛋白生产细胞,其中包含慢病毒质粒载体pLenti6.3 / TO / HA(H1N1)-M1-NA,其中包含流感病毒(H1N1)蛋白,血凝素,神经氨酸酶和M1基因,如图所示。 。 3.描述的是MDCK,流感病毒产生细胞,其包含慢病毒质粒载体pLenti6.3 / TO / HA(H3N2)-M1-NA,其包含来自H3N2亚型菌株的克隆的血凝素基因,以及神经氨酸酶(H1N1)亚型菌株的M1蛋白基因。 2.描述的是MDCK(一种流感病毒蛋白生产细胞),其中包含慢病毒质粒载体pLenti6.3 / TO / HA(B-)-M1-NA,其中包含B型流感病毒血凝素基因以及神经氨酸酶和(H1N1)流感病毒的M1蛋白基因,如图2所示。 1 .;效果:流感病毒MDCK产生细胞用于流感病毒的病毒样颗粒(HPV)发育。; 11 cl,23 dwg,6 ex,10 tbl

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