METHOD OF PRODUCING RABIES VIRUS ANTIGEN FOR SEROLOGICAL DIAGNOSIS

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to veterinary science, and can be used in preparing the rabies virus antigen for serological diagnosis. That is ensured by preparing a suspension of brain white mice experimentally infected with rabies virus. Brain tissue is homogenised in 0.1 M phosphate-buffer solution at pH 7.2–7.4. Obtained cerebral suspension is held in test tubes for at least 5 minutes until complete precipitation of silicon carbide balls. Supernatant is taken and 0.1 M phosphate-buffer solution is added to the test tubes, then shaken 3 times, held for at least 5 minutes and centrifuged for 50 minutes at 5,000 rpm. Supernatant is concentrated by ultracentrifugation at 37,000 rpm for 4 hours. Supernatant is then removed and the resulting resuspended residue is layered on a step gradient of 10–60 % sucrose. Ultracentrifugation for 3 hours at 30,000 rpm at temperature of +40 °C viral material is then fractionated by gel filtration. Immunoblot results are used to select active fractions, re-precipitate them with alcohol and obtain an antigen of rabies virus.;EFFECT: invention increases specificity and sensitivity of the obtained antigen and increases output of the active antigen.;1 cl, 1 tbl, 6 dwg