METHOD FOR ENTEROVIRUS GENOTYPING BY GENOME REGION 1A-1B SEQUENCING METHOD

摘要

FIELD: biology.;SUBSTANCE: invention relates to molecular biology, laboratory diagnostics, virology and epidemiology and aims at genotyping enterovirus by sequencing 1A-1B genome. Viral RNA is recovered, cDNA is obtained, which is used as a matrix in the reaction of "half-embedded" polymerase chain reaction. Genotyping of detected enteroviruses is performed by a method of comparative analysis of sequences obtained after amplicon sequencing, with sequences of genomes of enteroviruses from an international database of genetic data.;EFFECT: invention provides developing a more sensitive method of enterovirus genotyping.;1 cl