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METHOD FOR HUMAN GENOTYPE DETERMINATION BY MUTATION c.496AG IN 6 EXON OF DPYD GENE

机译：突变基因c.496A> G的6个外显子突变测定人类基因型的方法

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摘要

FIELD: biotechnology.;SUBSTANCE: invention refers to biotechnology, molecular biology and genetics. Disclosed is a method for determining a human genotype by mutation c.496AG in 6 exon of a gene DPYD, involving real-time PCR with detection of results using a method of analyzing melting curves (HRM) and optimizing PCR using an adapted buffer for Taq-polymerase of composition Tris-HCl 67 mM, (NH₄)₂SO₄ 166 mM, tween-20 0.1 %, glycerine 1 %, pH 8.7.;EFFECT: invention provides high sensitivity and specificity of the method for determining a human genotype by mutation c.496AG in 6 exon of a gene DPYD.;1 cl, 1 dwg, 3 ex

机译：领域：生物技术;实体：发明是指生物技术，分子生物学和遗传学。公开了一种通过在基因 DPYD的6个外显子中突变c.496A> G来确定人类基因型的方法，该方法涉及实时PCR并使用解链曲线分析方法（HRM）检测结果并使用适合于Tris-HCl 67 mM（NH _{4 ）_{2 SO _{4 166 mM的Taq聚合酶的缓冲液优化PCR吐温20 0.1％，甘油1％，pH 8.7 .;效果：本发明提供了通过在基因 DPYD 的6个外显子中突变c.496A> G来确定人类基因型的方法的高灵敏度和特异性> .; 1 cl，1 dwg，3 ex}}} 展开▼

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公开/公告号RU2701375C1

专利类型

公开/公告日2019-09-26

原文格式PDF

申请/专利权人 FEDERALNOE GOSUDARSTVENNOE BYUDZHETNOE UCHREZHDENIE NATSIONALNYJ MEDITSINSKIJ ISSLEDOVATELSKIJ TSENTR ONKOLOGII IMENI N.N. BLOKHINA MINISTERSTVA ZDRAVOOKHRANENIYA ROSSIJSKOJ FEDERATSII (FGBU NMITS ONKOLOGII IM. N.N. BLOKHINA MINZDRAVA ROSSII);
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申请/专利号RU20190108704

发明设计人 LYUBCHENKO LYUDMILA NIKOLAEVNA (RU);SEMYANIKHINA ALEKSANDRA VLADIMIROVNA (RU);POSPEKHOVA NATALYA IVANOVNA (RU);GOLOVINA DARYA ANDREEVNA (RU);SAFRONOVA VERA MIKHAJLOVNA (RU);MAMEDLI ZAMAN ZAUR OGLY (RU);MESHCHERYAKOV ANDREJ ALBERTOVICH (RU);FILIPPOVA MARGARITA GENNADEVNA (RU);STILIDI IVAN SOKRATOVICH (RU);
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申请日2019-03-26

分类号C12N15/09;

国家 RU

入库时间 2022-08-21 11:46:01

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