RECOMBINANT PROTEIN HAVING PROTECTIVE ACTION ON MENINGOCOCCUS (EMBODIMENTS), POLYNUCLEOTIDE CODING RECOMBINANT PROTEIN, RECOMBINANT PLASMID DNA CONTAINING SAID POLYNUCLEOTIDE, HOST CELL CONTAINING SAID RECOMBINANT PLASMID DNA, METHOD OF PRODUCING RECOMBINANT PROTEIN

摘要

FIELD: biotechnology.;SUBSTANCE: group of inventions relates to biotechnology. Disclosed is a recombinant protein having protective effect on meningococcus having amino acid sequence SEQ ID NO: 2, consisting of three fragments of primary structure of IgA protease Neisseria meningitidis serogroup B, connected in a single polypeptide chain. Also disclosed is a recombinant protein variant having the amino acid sequence SEQ ID NO: 4, consisting of two fragments of primary structure of IgA protease Neisseria meningitidis serogroup B, connected in a single polypeptide chain. Invention also discloses a polynucleotide encoding a corresponding recombinant protein, a recombinant plasmid DNA comprising a corresponding polynucleotide, a host cell transformed with recombinant plasmid DNA and producing a recombinant protein, and a method of producing a recombinant protein.;EFFECT: group of inventions provides producing highly purified immunogenic proteins with a high level of protective action on meningococci.;6 cl, 3 dwg, 2 tbl, 10 ex