Microscope and method for microscopy of a sample for displaying images with extended depth of field or three-dimensional images

摘要

The present invention initially relates to a method for microscopy of a sample (01) with a microscope. In a method step, microscopic individual images of different regions of the sample (01) are recorded, while a receiving region of the microscope is moved over the regions of the sample (01). The individual images of the respective region of the sample (01) are recorded with several different focus positions (02). Images with extended depth of field or three-dimensional images for each of the regions of the sample (01) are determined from the captured frames and displayed to the operator. As soon as the movement of the recording region over one of the regions of the sample (01) is stopped, further microscopic individual images of this region of the sample (01) with different focus positions (02) are recorded according to the invention. The further individual images are used to increase an axial and / or lateral resolution of the currently displayed image with extended depth of field or of the currently displayed three-dimensional image. Furthermore, the invention relates to a microscope with a lens for imaging a sample (01).