首页> 外国专利> METHOD FOR RAPID DETERMINATION OF SENSITIVITY OR RESISTANCE TO POLYMYXINS IN BACTERIA OF PSEUDOMONADACEAE AND MORAXELLACEAE FAMILIES (NON-FERMENTATIVE GRAM NEGATIVE BACTERIA)

METHOD FOR RAPID DETERMINATION OF SENSITIVITY OR RESISTANCE TO POLYMYXINS IN BACTERIA OF PSEUDOMONADACEAE AND MORAXELLACEAE FAMILIES (NON-FERMENTATIVE GRAM NEGATIVE BACTERIA)

机译:快速测定假单胞菌科和莫拉菌科细菌(非发酵革兰氏阴性菌)细菌中多粘菌素的敏感性或耐药性的方法

摘要

A method for the rapid determination of resistance or resistance to antibiotics of the polymyxin family, gram-negative non-fermentative bacteria of the Pseudomonadaceae and Moraxellaceae families, characterized in that it comprises: - an inoculum step of said bacteria in a liquid medium comprising at least: ▪ a yeast extract and polyethylene glycol sorbitan monooleate, and ▪ a colored pH indicator all constituting a liquid composition, then: - a step of contacting by any suitable means of said liquid composition with an antibiotic compound of the family of polymyxins, then - the incubation of the entire composition-liquid antibiotic compound, for a period of 2 to 4 hours, then the observation and evaluation during this same period or at the resulting from it, the color change of said set
机译:一种快速测定多粘菌素家族,假单胞菌科和莫拉氏菌科的革兰氏阴性非发酵菌的抗药性或耐药性的方法,其特征在于:-在液体培养基中所述细菌的接种步骤,该方法包括:至少:▪酵母提取物和聚乙二醇脱水山梨糖醇单油酸酯,和▪有色的pH指示剂,均构成液体组合物,然后:-通过所述液体组合物的任何合适方式与多粘菌素家族的抗生素化合物接触的步骤,然后-将整个组合物-液体抗生素化合物孵育2至4小时,然后在相同时期内或由此产生的观察和评估结果,所述组的颜色变化

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