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HIGH-THROUGHPUT SINGLE-CELL ANALYSIS COMBINING PROTEOMIC AND GENOMIC INFORMATION
HIGH-THROUGHPUT SINGLE-CELL ANALYSIS COMBINING PROTEOMIC AND GENOMIC INFORMATION
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机译:结合蛋白质组学和遗传学信息的高通量单细胞分析
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摘要
Disclosed herein are methods for single-cell sequencing. In some examples, the methods include enriching a sample comprising a plurality of cells for cells of interest to produce an enriched cell sample; isolating one or more cells of interest in the enriched cell sample; and obtaining sequence information of one or more polynucleotides from each of the one or more isolated cells. Obtaining sequence information may include generating a molecularly indexed polynucleotide library from the one or more isolated cells. Enriching the sample may include focusing cells of interest in the sample using acoustic focusing.
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