APPARATUS FOR HIGH-THROUGHPUT RAPID TRAPPING OF CIRCULATING TUMOR CELLS AND METHOD FOR PURIFYING CIRCULATING TUMOR CELLS

摘要

An apparatus for high-throughput rapid trapping and purifying of circulating tumor cells and a method for purifying circulating tumor cells, relating to the field of biotechnology. The apparatus for high-throughput rapid trapping of circulating tumor cells comprises: a filter module, the filter module comprises a filter, a filter membrane which is arranged inside the filter and sealed with an inner wall of the filter, and a negative pressure device; the negative pressure device is connected with an end of an outlet of the filter, and used for maintaining a negative pressure within the filter; when a negative pressure is maintained within the filter by the negative pressure device, the filter membrane is configured to trap circulating tumor cells, then stain and wash the trapped cells by using reagents which are automatically loaded by means of a reagent loading module. The apparatus may automatically control loading a sample liquid to the filter as well as the negative pressure device, such that the process in which the circulating tumor cells are trapped by using the filter membrane and then stained has the advantages of being highly automated and having a high throughput (which can be achieved by means of increasing the number of filters).