DETECTION OF IMMUNOGLOBULIN LIGHT CHAIN RESTRICTION BY RNA IN SITU HYBRIDIZATION

摘要

The invention provides a method for detecting immunoglobulin light chain restriction and clonality in B cells by RNA in situ hybridization assays on a sample of B cells from a subject. The detection is based on the pattern of signal associated with hybridized immunoglobulin kappa chain constant region (IGKCR) probe, immunoglobulin lambda chain constant region (IGLCR) probe, and immunoglobulin lambda-like polypeptide 5 (IGLL5) probe. The invention also provides a kit for conducting the RNA in situ hybridization assays.