TYPE I-E CRISPR-CAS SYSTEMS FOR EUKARYOTIC GENOME EDITING

摘要

Compositions and methods are provided for modification of a target sequence in the genome of a cell. The methods and compositions employ a cascade system and guide polynucleotide to provide an effective system for targeting, binding to, and modifying or altering target sequences within the genome of a cell or organism. Also provided are systems further comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one nuclease covalently or non-covalently linked to, or assembled with, at least one protein subunit of a cascade, and for compositions and methods for direct delivery of endonucleases.