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METHOD FOR DETERMINING PRESENCE OR ABSENCE OF FUNGUS BELONGING TO GENUSCERCOSPORA OR PSEUDOCERCOSPORA IN SAMPLE
METHOD FOR DETERMINING PRESENCE OR ABSENCE OF FUNGUS BELONGING TO GENUSCERCOSPORA OR PSEUDOCERCOSPORA IN SAMPLE
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机译:确定样品中存在或不存在真菌的类比真菌或假单胞菌的方法
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摘要
The present invention provides a method for determining whether or not a sample contains at least one of Cercospora fungus and Pseudocercospora fungus. In the method, first, the sample is added an aqueous solution containing N,N,N',N'-Tetramethyl-1,4-phenylenediamine and 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium to provide a mixture. The aqueous solution has a pH of not less than 5.1 and not more than 6.5. Then, the mixture is irradiated with light having a wavelength of 400 to 500 nanometers to measure a first absorbance Ay of the mixture, and the mixture is irradiated with light having a wavelength of 500 to 800 nanometers to measure a second absorbance Ax of the mixture. If the value of Ay / Ax is not less than 1.5 or the mixture is orangey, it is determined that the sample contains at least one of Cercospora fungus and Pseudocercospora fungus.
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