MICROFLUIDIC SYSTEM FOR DIGITAL POLYMERASE CHAIN REACTION OF A BIOLOGICAL SAMPLE, AND RESPECTIVE METHOD

摘要

A microfluidic system (1; 1') for dPCR of a biological sample and a respective method is provided by the present invention, the system (1; 1') comprising at least one microfluidic device (2) having an inlet (23), an outlet (24), a flow channel (25) connecting the inlet (23) to the outlet (24), and an array of reaction areas (26) in fluidic communication with the flow channel (25), a flow circuit (3) connectable to the microfluidic device (2), for flowing liquid through the flow channel (25) of the microfluidic device (2), a sample liquid source connectable to the microfluidic device (2), for providing the microfluidic device (2) with a sample liquid (27), a primary sealing liquid source connectable to the microfluidic device (2), for providing the microfluidic device (2) with initial sealing liquid (28) for sealing the sample liquid (27) inside the array of reaction areas (26), a secondary sealing liquid source (4) connectable to the microfluidic device (2), for providing the microfluidic device (2) with additional sealing liquid (29), and a pumping means (31) connected to the flow circuit (3) and adapted to pump said additional sealing liquid (29) through the flow channel (25).