SUBTILISIN VARIANTS HAVING A MUTATION IN THE S2 OR S2' POCKET

摘要

The invention relates to a subtilisin BPN' variant or homologue thereof, comprising the following mutations compared to subtilisin BPN' represented by SEQUENCE ID NO: 2 or a homologue sequence thereof: - a deletion of the amino acids corresponding to positions 75-83; - a mutation at the amino acid position corresponding to S221, the mutation corresponding to S221C or S221selenocysteine, preferably S221C; - at least one further mutation selected from the group consisting of amino acid positions corresponding to F189W, F189Y, S33D, S33T, N218D, N218T, N218E, N62D, N62S, N62W, and N62Y; preferably a mutation at the amino acid position corresponding to P225; wherein the amino acid positions are defined according to the sequence of subtilisin BPN' represented by SEQUENCE ID NO: 2. The invention further relates to enzymatically synthesizing a peptide.