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SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION
SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION
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机译:自我检测酶活性的问题
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摘要
Provided is a compound comprising the structure: (SIG)-(SI-MOD)m. In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.
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机译:提供了一种具有以下结构的化合物:(SIG)-(SI-MOD) m Sub>。在该化合物中,SIG是信号传导分子,SI是与SIG结合的自消灭结构,因此SIG相对于没有SI的SIG信号具有降低的信号,MOD是与SI结合的部分,该部分容易被修饰激活剂,m为1至约10的整数。使用该化合物,当通过激活剂修饰MOD时,SI不稳定并自SIG裂解,从而SIG产生增强的信号。还提供了一种使用上述化合物确定样品是否包含活化剂的方法。另外提供了一种使用上述化合物(其中硝基还原酶是激活剂)来确定细胞是否包含硝基还原酶的方法。还提供了一种使用上述化合物(其中硝基还原酶是激活剂)来确定哺乳动物细胞是否缺氧的方法。还提供了一种使用上述化合物(其中硝基还原酶是活化剂)来检测包含硝基还原酶的微生物的方法。还提供了一种使用上述化合物(其中硝基还原酶是活化剂)来鉴定样品中硝基还原酶的方法。
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