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MULTIPLEXED IMAGING OF TISSUES USING MASS TAGS AND SECONDARY ION MASS SPECTROMETRY
MULTIPLEXED IMAGING OF TISSUES USING MASS TAGS AND SECONDARY ION MASS SPECTROMETRY
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机译:利用质谱标签和二次离子质谱法对组织进行多重成像
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摘要
A method of generating a high resolution two-dimensional image of a sample comprising cells and extracellular structures is provided. In certain embodiments, the method comprises: labeling a sample with at least one mass tag, thereby producing a labeled sample; scanning the sample with a secondary ion mass spectrometer (SIMS) ion beam to generate a data set that comprises spatially-addressable measurements of the abundance of the mass tag across an area of the sample; and outputting the data set. In many embodiments, the data set contains the identity and abundance of the mass tag. A system for performing the method is also provided.
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