PROBLEM TO BE SOLVED: To provide a culture method and apparatus capable of improving biological activity, a wastewater treatment method and apparatus. A method for culturing cells using a medium, wherein the concentration of at least one of Mn, Mo and Co among the metal elements contained in the medium is 0.001 mg / L or less. The method. Further, a metal element removing unit 14 that removes a metal element from the medium, and a metal element adding unit 16 that adds a metal element that does not contain Mn, Mo, and Co to the medium from which the metal element has been removed by the metal element removing unit 14. The metal element addition unit 16 includes a culture unit 18 for culturing cells using a medium to which a metal element containing no Mn, Mo, and Co is added. Further, it is a wastewater treatment method and apparatus for performing wastewater treatment by adding metal elements containing no Mn, Mo and Co to the wastewater discharged from the production facility 46 using water from which the metal elements have been removed. [Selection diagram] Fig. 4
展开▼