METHOD FOR MASS-PRODUCING NATURAL KILLER CELLS AND USE OF NATURAL KILLER CELLS OBTAINED THEREBY AS ANTICANCER AGENT

摘要

PROBLEM TO BE SOLVED: To provide a method for mass-producing natural killer cells and use of the natural killer cells as an anticancer agent.;SOLUTION: A method for producing fresh NK (natural killer) cells is provided, the method comprising the steps of: 1) obtaining CD3-negative cells by removing CD3-positive T cells from monocytes; and 2) mixing the CD3-negative cells with IL-15 and IL-21 for cultivation after that, wherein the step 1) is performed by allowing the CD3-positive T cells to crosslink to erythrocytes and then isolating the CD3-negative cells by density-gradient in centrifugation. The fresh NK cells, cold-preserved NK cells and cryopreserved NK cells thus produced can exhibit therapeutic effects against various cancers, including colorectal cancer, lung cancer, liver cancer, pancreatic cancer and leukemia, and can be effectively used as cellular therapeutic agents.;SELECTED DRAWING: Figure 7a;COPYRIGHT: (C)2020,JPO&INPIT