The agent for promoting muscle differentiation according to the present invention includes an oligoDNA having an activity of promoting muscle differentiation by being applied to a cell or an individual. The oligo DNA preferably contains a core base sequence represented by 5'TTAGGG3 'or 5'TGAGGGG3', and has a base sequence AGA or a base sequence AAG at the 5 'end. Further, berberine or a compound thereof or a salt thereof may be contained in combination with the oligo DNA.
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