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Systems and methods for high-speed wide-field illumination scanning for in vivo small animal fluorescence tomography
Systems and methods for high-speed wide-field illumination scanning for in vivo small animal fluorescence tomography
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机译:用于体内小动物荧光层析成像的高速宽场照明扫描系统和方法
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摘要
Presented herein are systems and methods for tomographic imaging that provide rapid illumination of multiple excitation sites over a wide field of view with one or more excitation light beams from one or more excitation sources. .. The technique described herein utilizes a galvano optical scanner to scan the excitation light beam through multiple locations across the scanning area corresponding to the field of view being imaged. In certain embodiments, the systems and methods described herein utilize specially tuned shaped excitation light beams to maintain a small spot size over a wide scanning area. The ability to scan an entire large area while still maintaining the small spot size provided by the techniques described herein allows accurate, high resolution tomography of large or multiple subjects, thereby. Extend the performance of tomographic imaging.
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