Fast and efficient glycoprotein deglycosylation

摘要

The present invention is a rapid and cost-effective method of deglycosylating glycoproteins, comprising anionic surfactants and reducing agents and nonionic surfactants to obtain glycoproteins with stable, modified glycoproteins. The method is disclosed in combination with an agent. In addition, endoglycosidase is added to the denatured glycoprotein to cleave N-linked glycans to obtain deglycosylated proteins. A rapid tool for assessing protein conformation due to partial deglycosylation is also presented, where partially deglycosylated proteins are analyzed using capillary electrophoresis (CE-SDS). You.