Fermentation medium and culture method of L-isoleucine-producing Corynebacterium glutamicum

摘要

A basal medium and a growth factor, wherein the growth factor is composed of choline, betaine and vitamin B6, and the content of each component in the fermentation medium is 0.2 to 1 g / L of choline and 0.25 to 0.5 mg / L of betaine. A fermentation medium for L-isoleucine-producing Corynebacterium glutamicum having a vitamin B6 content of 0.05 to 0.3 mg / L. L-isoleucine-producing Corynebacterium glutamicum is inoculated into the fermentation medium such that the volume of the bacterial solution occupies 5 to 20% of the volume of the fermentation medium, the pH is adjusted to 6.5 to 7 with aqueous ammonia, and dissolved oxygen is added. Is controlled to 30 to 50% and fermented for 25 to 30 h, then the dissolved oxygen is reduced to 15 to 25%, and the 50-80% glucose solution is added to the fermentation liquor so that the residual sugar is 3 to 4%. A method of fermenting and culturing L-isoleucic acid-producing Corynebacterium glutamicum, in which the fermentation is stopped by pouring, followed by fermenting for 60 to 70 hours, and controlling the temperature of the entire fermentation process to 29 to 33 ° C.