Mass spectrometry assay method for detection and quantification of renal function metabolites

摘要

According to mass spectrometry, N-acetylthreonine, TMAP, phenylacetylglutamine, tryptophan, creatinine, meso-erythritol, arabitol, myo-inositol, N-acetylserine, N-acetylalanine, 3-methylhistidine, trans- A method for determining the amount of one or more analytes selected from the group consisting of 4-hydroxyproline, kynurenine, urea, C-glycosyltryptophan, 3-indoxyl sulfate, pseudouridine, and combinations thereof. It has been described. The method comprises subjecting the sample to an ion source under conditions suitable to generate one or more ions detectable by mass spectrometry from each of one or more of these analytes; Determining the amount of one or more ions from each of the species or analytes by mass spectrometry; using the measured amounts of the one or more ions to determine one or more of the one or more ions in the sample Determining the amount of each of the analytes. Also described is a kit comprising one or more isotopically labeled analogs as an internal standard for each of the one or more analytes.