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Method for detecting cancer cells using living cells

机译:利用活细胞检测癌细胞的方法

摘要

The purpose of the present invention is to provide a novel cancer cell detection method that uses living body-derived cells and that can be used even in cytodiagnosis. In particular, the purpose of the present invention is to provide a novel cancer cell detection method that makes it possible to perform imaging of cells in a living state and a dual detection method for cancer cells in which the aforementioned method is combined with a pre-existing dyeing method for cytodiagnosis. Provided is a cancer cell detection method that uses living body-derived cells and that includes: incubating living cells included in a sample taken from a person together with a fluorescently-labeled L-glucose derivative and detecting the fluorescently-labeled L-glucose derivative that is taken up into the cells; and detecting fluorescence emitted by the L-glucose derivative that is present within the cells while the cells are attached to a thin glass or plastic plate. Also provided is a dual detection method for cancer cells in which the cancer cell detection method that uses living body-derived cells is combined with a dyeing method using cells that are fixed using an alcohol or the like.
机译:本发明的目的是提供一种使用活体来源的细胞并且甚至可以用于细胞诊断中的新颖的癌细胞检测方法。特别地,本发明的目的是提供一种新颖的癌细胞检测方法和一种双重检测方法,该方法使得能够对处于存活状态的细胞进行成像,并且该方法将上述方法与预现有的用于细胞诊断的染色方法。本发明提供一种癌细胞检测方法,其使用来自生物体的细胞,该方法包括:将从人采集的样品中包含的活细胞与荧光标记的L-葡萄糖衍生物一起进行孵育,并检测荧光标记的L-葡萄糖衍生物。被吸收到细胞中;当细胞附着在薄玻璃或塑料板上时,检测细胞内存在的L-葡萄糖衍生物发出的荧光。还提供了一种用于癌细胞的双重检测方法,其中将使用活体来源的细胞的癌细胞检测方法与使用通过酒精等固定的细胞的染色方法相结合。

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