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Method for detecting thyroid cancer by enzyme activity staining of CD26 / DPPIV and detection kit using the same

机译:CD26 / DPPIV酶活性染色检测甲状腺癌的方法及使用该试剂盒的检测试剂盒

摘要

PROBLEM TO BE SOLVED: To provide a detection method of thyroid cancer and detection kit using the method.SOLUTION: A detection method of a thyroid cancer cell by enzyme activity staining of CD26/DPPIV (dipeptidyl peptidase IV) includes: a process of fixing a target specimen to be detected by fixing liquid; and a process of allowing the fixed specimen to react with reaction liquid and detecting a cancer cell, in which the reaction liquid for allowing the specimen to perform reaction is reaction liquid formed by mixing phosphate buffer, GPMN (Glycyl-prolyl-4-methoxy-β-naphthylamide) substrate liquid of N-N dimethylformamide solvent, FBB (Fast Blue B salt) pigment solution using solvent such as tert-butyl alcohol and acetone.SELECTED DRAWING: Figure 2
机译:解决的问题:提供一种甲状腺癌的检测方法和使用该方法的检测试剂盒。解决方案:通过CD26 / DPPIV(二肽基肽酶IV)的酶活性染色检测甲状腺癌细胞的方法包括:通过固定液体检测目标样品;以及使固定试样与反应液反应并检测癌细胞的方法,其中,使试样进行反应的反应液是通过混合磷酸盐缓冲液GPMN(Glycyl-prolyl-4-甲氧基- NN二甲基甲酰胺溶剂的β-萘甲酰胺底物液,使用叔丁醇和丙酮等溶剂的FBB(蓝蓝色B盐)颜料溶液。选择的图:图2

著录项

  • 公开/公告号JP6612530B2

    专利类型

  • 公开/公告日2019-11-27

    原文格式PDF

  • 申请/专利权人 社会医療法人同心会;

    申请/专利号JP20150110768

  • 发明设计人 荒武 八起;清山 和昭;

    申请日2015-05-29

  • 分类号C12Q1/37;

  • 国家 JP

  • 入库时间 2022-08-21 11:32:59

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