Method for determining undifferentiated state of pluripotent stem cells, method for subculturing pluripotent stem cells, and device used in those methods

摘要

The present disclosure is a method for determining the undifferentiated state of pluripotent stem cells, which comprises irradiating a test culture medium for culturing pluripotent stem cells with light having a wavelength in the range of 190 nm to 2500 nm or a partial range thereof. , Its reflected light, transmitted light or transmitted reflected light is detected to obtain absorbance spectrum data, based on an analytical model created in advance using multiple control culture media used for culturing pluripotent stem cells, the absorbance spectrum It comprises determining the undifferentiated state of pluripotent stem cells by analyzing the absorbance of all wavelengths measured in the data or a partial range thereof, wherein the plurality of control culture media are A medium used to maintain the differentiated state, and one or more types of differentiation selected from the group consisting of an ectodermal cell differentiation induction medium, a mesodermal cell differentiation induction medium, and an endoderm cell differentiation induction medium A method comprising an induction medium is provided.