Super-resolution observation apparatus and super-resolution observation method

摘要

PROBLEM TO BE SOLVED: To enable performance in STED microscopes to be further enhanced.SOLUTION: A super-resolution observation device comprises: an illumination optical system that converges excitation light 111 for performing a multiphoton excitation of a substance contained in an object 20 toward the object, and converges induction light 112 for inducing and discharging the object in the excitation toward an area where the converging area of the excitation light partially overlaps; a modulation part 29 that modulates characteristics of the excitation light bounding toward the object in a time direction; and a detection part 23 that detects a time-varying component with a N-time frequency of modulated frequencies (where N is a natural number equal to 1 or more) out of fluorescence naturally discharged by the object existing in the converging area of the excitation light and the induction light.