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Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
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机译:蛋白质-寡核苷酸结合物和蛋白质-寡核苷酸结合物的生物催化方法和组合物
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摘要
A composition comprising a polypeptide ligated to an oligonucleotide through a sterol linker. A method of ligating a polypeptide to an oligonucleotide, comprising a polypeptide having a hedgehog steroyl transferse catalytic domain at the C-terminal of the polypeptide with an electrophilic residue, e.g., glycine, between polypeptide and the hedgehog steroyl transferse catalytic domain, and a steroylated oligonucleotide in solution, and permitting a reaction to cleave the hedgehog steroyl transferse catalytic domain from the polypeptide while ligating the steroylated oligonucleotide to the glycine at the C-terminal of the polypeptide. The oligonucleotide may be, for example, a therapeutic, diagnostic, or affinity ligand.
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