Method for separating flavonoid substances in Camellia nitidissima Chi based on a magnetic nanoparticles-PAMAM nano composites

摘要

The present invention discloses a method for separating flavonoid substances in Camellia nitidissima Chi based on a magnetic nanoparticles-PAMAM nano composites, which comprises the following steps: preparing PAMAM dendrimer, then using the PAMAM dendrimer to prepare the magnetic nanoparticles-PAMAM nano composites, then adding the obtained magnetic nanoparticles-PAMAM nano composites in a Camellia nitidissima Chi extract, extracting and performing magnetic separation on the flavonoid substances in Camellia nitidissima Chi under ultrasound or microwave condition. According to the present invention, flavonoid substances with faintly acid characteristics are extracted and adsorbed in a plant concentrate such as Camellia nitidissima Chi or Hedyotis diffusa etc. based on the magnetic nanoparticles-PAMAM nano composites, in a successive step, high efficiency separation of the flavonoid substances can be realized by the technologies such as magnetic separation and microwave-assisted extraction.