Microscopic imaging system and method with three-dimensional refractive index tomography

摘要

Microscopic imaging system and method with three-dimensional refractive index tomography are provided. The microscopic imaging system includes: an illumination providing module, configured to provide a beam of parallel lights with a modulated intensity; a microscopic sample, arranged at downstream of the illumination providing module, and configured to modulate a phase of the beam of parallel lights, such that emergent lights passing through the microscopic sample carry information of a three-dimensional refractive index field of the microscopic sample; a microscopic imaging module, arranged at downstream of the microscopic sample, and configured to form an image by using the emergent lights; and a controlling module, configured to process the image to reconstruct three-dimensional refractive index information of the microscopic sample.