A method of producing a preparation of embryonic DNA that can be performed without risking damage to a developing embryo is described. A sample of cell-free media in which an embryo was grown in vitro from the 4-blastomeres stage to the blastocyst stage of development is obtained, wherein the sample comprises cell-free embryonic DNA (cfeDNA). cfeDNA is obtained from the sample and recovered. These steps can be carried out non-invasively, without performing a biopsy of the embryo. The method can be used for optimizing the likelihood of a live birth after implantation of human egg fertilized in vitro, and for reducing the risk of damage to a normal embryo that has been fertilized in vitro.
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