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Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression
Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression
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机译:使用阻遏物多肽和诱导型启动子进行基因表达的宿主细胞和方法
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摘要
The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
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