METHODS AND COMPOSITIONS FOR POLYMERASE II (POL-II) BASED GUIDE RNA EXPRESSION

摘要

Compositions and methods are provided for editing nucleotides and/or altering target sites in the genome of a cell. The methods and compositions employ a recombinant DNA construct comprising a Pol-II promoter operably linked to a polynucleotide encoding a single guide RNA, wherein said guide RNA is capable of forming a guide RNA/Cas endonuclease complex, wherein said complex can bind to and cleave a target site sequence in the genome of a cell such as a eukaryote cell. The present disclosure further describes methods and compositions employing said recombinant DNA construct to modify the genome of non-conventional yeast.