There is described herein a mutant plant or part thereof having at least partially reduced expression or activity of at least two heavy metal ATPases (HMAs), said two HMAs comprising, consisting or consisting essentially of: (i) polypeptides having at least 65% sequence identity to SEQ ID NO:1 and SEQ ID NO:2; (ii) polynucleotides encoding the polypeptides set forth in (i); or (iii) polynucleotides having at least 65% sequence identity to SEQ ID NO:3 and SEQ ID NO:4 encoding HMAs; wherein the expression or activity of one of the HMAs set forth in (i) or (ii) or (iii) is partially reduced or lost and the expression or activity of one of the HMAs set forth in (i) or (ii) or (iii) is lost as compared to a control plant; and wherein the mutant plant or part thereof exhibits at least a 27% reduction, as compared to the control plant, in the accumulation of cadmium in leaf when the mutant plant is grown in the field in the presence of naturally or non-naturally occurring cadmium.
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机译:本文描述了具有至少两种重金属ATP酶(HMA)的表达或活性至少部分降低的突变植物或其部分,所述两种HMA包含或基本由以下组成:(i)具有至少65%序列的多肽与SEQ ID NO:1和SEQ ID NO:2相同; (ii)编码(i)中列出的多肽的多核苷酸; (iii)与编码HMA的SEQ ID NO:3和SEQ ID NO:4具有至少65%的序列同一性的多核苷酸;或其中(i)或(ii)或(iii)中所述的一种HMA的表达或活性被部分降低或丢失,并且(i)或(ii)或中所述的一种HMA的表达或活性被还原。 (iii)与对照工厂相比损失;并且其中当所述突变植物在天然或非天然存在的镉存在下生长时,与对照植物相比,所述突变植物或其部分与叶中的镉积累相比显示至少27%的减少。 。
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