There is described herein a mutant plant or part thereof having at least partially reduced expression or activity of at least two heavy metal ATPases (HMAs), said two HMAs comprising, consisting or consisting essentially of: (i) polypeptides having at least 65% sequence identity to SEQ ID NO:1 and SEQ ID NO:2; (ii) polynucleotides encoding the polypeptides set forth in (i); or (iii) polynucleotides having at least 65% sequence identity to SEQ ID NO:3 and SEQ ID NO:4 encoding HMAs; wherein the expression or activity of one of the HMAs set forth in (i) or (ii) or (iii) is partially reduced or lost and the expression or activity of one of the HMAs set forth in (i) or (ii) or (iii) is lost as compared to a control plant; and wherein the mutant plant or part thereof exhibits at least a 27% reduction, as compared to the control plant, in the accumulation of cadmium in leaf when the mutant plant is grown in the field in the presence of naturally or non-naturally occurring cadmium.
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机译:本文描述了具有至少两种重金属ATP酶(HMAS)的至少部分减少的突变植物或其活性,所述两个HMA包括:(i)具有至少65%序列的多肽组成或组成 SEQ ID NO:1和SEQ ID NO:2的身份; (ii)编码(i)中所述多肽的多核苷酸; (III)与SEQ ID NO:3和SEQ ID NO:4编码HMA的至少65%序列同一性的多核苷酸; 其中(i)或(iii)或(iii)中所述的HMS中的一种的表达或活性部分地减少或丢失,并且(i)或(ii)或(ii)中所述的HMA中的一种表达或活性 (iii)与对照植物相比丢失; 其中,与对照植物相比,突变植物或其一部分表现出至少27%的减少,与对照植物相比,当在天然或非天然存在的镉存在下突变植物在叶片中生长叶片时 。
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