SERUM-FREE CULTURE MEDIUM FOR LIMBAL STEM CELLS AND CULTURE METHOD THEREOF

摘要

A serum-free culture medium for limbal stem cells and a culture method thereof, wherein the serum-free culture medium includes a basic medium and supplements; wherein the supplements include: human recombinant EGF, insulin, 3,3′,5-triiodo-L-thyronine, hydrocortisone, forskolin, manganese sulfate monohydrate, sodium selenite, sodium metasilicate, ammonium metavanadate, nickel chloride hexahydrate, stannous chloride dihydrate, ethanolamine, O-phosphorylethanolamine, ammonium molybdate tetrahydrate, 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid, vitamin C, bovine serum albumin, lipid concentrate and serum substitute. The serum-free culture medium according to the present invention is free of fetal bovine serum and any animal-derived ingredient, can provide necessary adequate nutrition and good environment for cell growth and proliferation, effectively replaces the role of serum, realizes favorable cell growth, improves the cell purity and stability, provides quick and stable cell sources for researches on the mechanism of limbal stem cell specificity and transplantation therapy, and has broad clinical application prospects.