transformed leukocyte lineage and its biotechnological applications

摘要

transformed leukocyte lineage and its biotechnological applications. there are several methods for immortalizing primary cells, particularly human ones, for experimental and biotechnological purposes. Among the ways of obtaining immortalized cells, gene manipulation is commonly used in mechanisms involved in reversing cellular senescence, with the main target of enzymes active in the synthesis of the telomer, among other approaches. in the present development, there is a simple, easy to perform and reproducible and low-cost process: primary leukocyte cells obtained from peripheral blood are cultured in a medium for cells in suspension, plus an equivalent volume of supernatant from hut-78 cultures. . the resulting leukocyte lineage presents continuous division in an appropriate medium, a characteristic property of immortalization, in addition to the cells presenting an altered phenotype, with typical blast morphology and production of several cytokines and chemokines under any chemical stimulus. these cells can easily multiply on a large scale and their products can be fractionated and purified for industrial and pharmaceutical purposes, such as for the production of antibodies, virions, peptides and viral proteins.