MICRORGANISM HOSTING CELL UNDERSTANDING MODIFIED THYOSTERASE ENZYME AND CELL CULTURE UNDERSTANDING THE CELL

摘要

The present invention relates to novel mutant thioesterase enzymes and equivalents of these that occur in nature, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that exhibit altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and / or altered proportional productivity in product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells containing such polynucleotides. The invention additionally provides novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.