processes for increasing the yield of a r-methug-csf pegylation reaction process for gram-scale production of peg-r-methug-csf

摘要

improved protein pegylation process the present invention relates to a process for increasing the yield of the r-methug-csf pegylation reaction comprising conjugating r-methug-csf to an aldehyde peg in a free amine moiety at the n-terminus of the g-csf in the presence of a reducing agent in a pegylation buffer solution comprising a polyol having the formula cnh2n + 2on where it ranges from 3 to 6, or a carbohydrate, or a derivative thereof where the concentration of said polyol or carbohydrate or derived from it varies in the range of o, 1% to 10% w / w.