cell-free in vitro process for the production of a closed linear deoxyribonucleic acid (dna)

摘要

in vitro cell free process of producing a closed linear deoxyribonucleic acid (dna), kit, method of inducing immunological reaction against an antigen in a host, process of making a pharmaceutical composition and use of a closed linear dna molecule in the The manufacture of a medicament. An in vitro closed linear deoxyribonucleic acid (dna) production process comprises (a) contacting a dna model comprising at least one protelomerase target sequence with at least one dna polymerase in the presence of one or more primers under conditions that promote the amplification of the mentioned model; and (b) contacting amplified DNA produced in (a) with at least one protelomerase under conditions that promote closed linear DNA formation. A kit provides the necessary components in the process.