METHODS AND CONDITIONS FOR THE OBJECTIVE MODIFICATION OF THE GENERAL

摘要

Compositions and methods shall be provided to modify the genotype site of interest in a eukaryotic cell,a mammalian cell,a human or non-human mammalian cell,using a targeting vector (LTVEC) that includes various endogenous and exogenous nucleic acid sequences,as described in this. Further methods combine the use of LTVEC with a CRISPR/Cas system. Compositions and methods for:a the production of a genetically modified non-human animal containing one or more targeted changes in its germination line.