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PROMOTIONS FOR THE HURT PREPARATION OF MARKET GLYCOSYLAMINES AND FOR THE ANALYSIS OF GLYCOSYLLED BIOMOL TYPES GIVING THE SAME BACKGROUND
PROMOTIONS FOR THE HURT PREPARATION OF MARKET GLYCOSYLAMINES AND FOR THE ANALYSIS OF GLYCOSYLLED BIOMOL TYPES GIVING THE SAME BACKGROUND
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机译:促进市场上糖胺的伤害制备和分析相同背景的糖化生物酚类型的促进
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摘要
Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent. The derivizated glycosylamines can be separated from the reaction mixture and detected by chromatographic detection, fluorescence detection, mass spectrometry (“MS”), or Ultra Violet (“UV”) detection and/or a combination thereof.
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